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The same needle specifications apply: 30-31 gauge, 4-6mm length, inserted at 90 degrees with a gentle skin pinch

doi: 10.1002/poh2.61 32

J Plant Physiol 176:192201

In the detection of GST activity in cells, the cell number must be between 3-5 x 106, and the extraction of GST in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate